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KMID : 0613820170270101207
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Journal of Life Science
2017 Volume.27 No. 10 p.1207 ~ p.1214
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Inhibitory Effects of Schisandrae Fructus Ethanol Extract on the Production of Matrix Metalloproteinases in in vitro and in vivo Osteoarthritis Models
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Jeong Jin-Woo
Lee Hye-Hyeon
Kim Hong-Jae
Lee Ki-Won
Kim Ki-Young
Kim Sung-Gu
Hong Su-Hyun
Kim Bum-Hoi
Park Cheol
Choi Yung-Hyun
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Abstract
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Schisandrae Fructus (SF), the fruit of Schisandra chinensis (Turcz.) Baill., is widely used in traditional medicine for the treatment of a number of chronic diseases. SF extracts have been recently reported to attenuate the inflammatory responses in SW1353 human chondrocyte cells in in vitro and monosodium iodoacetate (MIA)-induced cartilage degradation in in vivo osteoarthritis (OA) models. However, their protective and therapeutic potentials against OA in primary culture chondrocytes and animal models remain unclear. Therefore, we investigated the effects of the ethanol extract of SF on the activity of matrix metalloproteinases (MMPs), biomarkers for diagnosis of OA, on interleukin (IL)-1β-induced primary cultured rat cartilage chondrocytes and MIA-induced osteoarthritis in a rat model. Our data indicated that SF treatment significantly reduced the mRNA expression and enzyme activity of MMP-1, -3 and -13 in IL-1β-induced primary cultured rat cartilage chondrocytes. The chondro-protective effects of SF were then analyzed in a rat OA model using a single intra-articular injection of MIA in the right knee joint. According to our results, the elevated levels of MMP-1 and -3 were markedly ameliorated by SF administration. Collectively, these findings indicate that SF could be a candidate for the treatment of OA.
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KEYWORD
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Interleukin-1β, matrix metalloproteinase, monosodium iodoacetate, osteoarthritis, Schisandrae Fructus (SF)
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